Device

Part:BBa_K1903012:Design

Designed by: Sofía Vieto Fonseca   Group: iGEM16_TEC-Costa_Rica   (2016-10-14)


PA1-RBS + IntC DnaB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Intein terminal C Ssp DnaB BBa_K1903011 was fused to an Anderson promoter BBa_J23101 associated to a RBS Elowitz BBa_B0034.


We assembled this device and verified it in an agarose gel electrophoresis:


We were able to express the Intein C with a size of 18KDa:

Source

References